Effect of hypoglycin on alanine release by skeletal muscles in vitro.

chrome P-450 concentration shows the disappearance to be loss of the active conformation of the enzyme rather than inhibition of an enzyme assay, as is employed with most other enzymes. No cytochrome P-450 could be detected by our methods in yeast strains N.C.Y.C. 73,74,366,379,533,686,692 and 716. A respiratory-deficient mutant of yeast (N.C.Y.C. 240) was shown to produce cytochrome P-450 even in 0. 1%-glucosecontaining media, where none is normally produced. The mutant tested produced 2.3nmol of cytochrome P-45Olg wet wt. of yeast in 0.1% glucose (and 5.5nmol in 20% glucose). The mutant does not produce cytochrome a + a , even in 0.1%glucose media. The cyclic AMP concentrations here have yet to be investigated.